Fig 2: AVPR1A protein expression by western blottingThe loading quantity of protein sample was 30 µg. Blank indicates that the cells were transfected with 100 ng ssEGFP miRNA. Lane 1: AVPR1A rs11174811 CCVE type HUVEC line, lane 2: AVPR1A rs11174811 CA/AA genotype HUVEC line, lane 4 and 6: AVPR1A rs3803107 GG genotype HUVECs. Lane 5 and 7: AVPR1A rs3803107 GA/AA genotype of HUVEC line.

Fig 3: Correlation between AVPR1A 3′ UTR SNPs and plasma miRNA levels(A) rs11174811 locus genotype and plasma miR-526b levels, 725 cases with CC genotype and 98 cases with CA/AA; (B) rs3803107 locus genotype and plasma miR-375 levels, 569 cases with GG genotype and 281 cases with CA/AA; (C) rs3803107 locus genotype and plasma miR-186 levels, 569 cases with GG genotype and 281 cases with CA/AA.

Fig 4: AVPR1A mRNA expression according to rs11174811 and rs3803107 genotypes(A) AVPR1A mRNA expression level in cells with different genotypes at rs11174811 locus. (B) AVPR1A mRNA expression level in cells with different genotype at rs3803107 locus. (C) The expression level of AVPR1A in cells transfected with miRNA-526b and control; (D) the expression level of AVPR1A in cells transfected with miRNA-375 and control; (E) the expression level of AVPR1A in the cells transfected with miRNA-186 and control.